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By showing how compounds conformed to the laws of constant, multiple, and equivalent proportions as well as to a series of semiempirical rules devised to cover specific classes of compounds, Berzelius established the quantitative specificity by which substances combined.
Using a quantitative specificity assay, we found that these Fabs are highly specific, possibly due to the alternate codon design we used to avoid consecutive arginines in the Fab interface.
In addition, the present study provides the first quantitative specificity data for over seventy residue/position combinations not covered by published substation library data.
Thus we generated data with the depth and quantitative specificity of RNA-seq, at a high temporal resolution, for cells grown by a common laboratory standard.
For example it becomes even more evident that although BoBac might have similar numbers of false-positives as the BacCow assay (qualitative specificity) the concentrations of BoBac in false-positive nontarget samples are much higher than those of BacCow (quantitative specificity).
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Despite recent progress in the development of high-throughput technologies for the measurement of protein-DNA interaction parameters as proposed by Maerkl and Quake et al. [4] and microarray based technologies for the analysis of TF binding specificities [5], [6], the determination of highly resolved quantitative binding specificity information remains laborious.
Because of its quantitative precision, specificity, and compatibility with high throughput robotic operations, the LOA assay eliminates bottlenecks in ES cell screening and mouse genotyping and facilitates maximal speed and throughput for knockout mouse production.
Based on the SVR model, a framework is implemented that allows to transfer and predict quantitative binding specificity data between TFs.
To train our approach a sufficient number of TFs has to be collected for which quantitative binding specificity information, the protein sequence and the DNA-binding domain annotation is available.
The conditions used for real-time PCR are as follows: 15 min at 95°C, followed by 35 cycles of 94°C for 30 s, 58°C for 30 s and 72°C for 30 s and a final extension at 72°C for 10 min. Quantitative and specificity (melting curve) analyses of amplified PCR products were carried out using associated software in the Opticon system (Opticon monitor3) by following the manufacture's protocol.
For instance, when comparing BacR and BoBac in this study, BacR showed higher quantitative source-specificity while BoBac exhibited higher quantitative source-sensitivity (marker abundance in target samples).
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