In general: In some of their experiments, the authors have used TLC and a (semi-quantitative) quantification of TLC to assess peripheral fat.
This methodology allows qualitative macroscopic assessment with Masson Trichrome staining [29] and quantitative morphometry (quantification of CVF) with picrosirius red [30] (Fig. 3).
Despite the recent development of real-time quantitative PCR, quantification of astroviruses in sewage had not been available up to now.
In this work we show that, by using a combination of external concentration referencing and a properly designed sample preparation optimized for quantitative determinations, quantification of active principles in pharmaceutical formulations can be performed with both speed and precision.
Equimolar pools of RNA-seq libraries were made following quantitative PCR quantification using a Kapa Library Quantification kit (KK4823, Kapa Biosystems).
The advantage of using a positron-emitting isotope is that PET is inherently quantitative, whereas quantification with, especially planar, gamma camera imaging involves considerable uncertainties that can often surpass estimated activity concentrations themselves.
The advantage of using a positron-emitting isotope is that PET is inherently quantitative, whereas quantification with planar imaging involves considerable uncertainties that can often be greater than estimated activity concentrations.
Primer sequences used for quantitative PCR quantification with SYBR Green (Invitrogen, Life Technologies, Paisley, UK) are listed in Supplemental Table 1, published on The Endocrine Society's Journals Online web site at http://endo.endojournals.org.org
The final double-stranded DNA libraries were quantitated via quantitative (q PCR using Library quantification kit Roche 454 Titanium (KAPA Biosystems, Boston, MA) prior to emulsion PCR amplification.
