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Binding affinities between proteins were measured by biolayer interferometry (Octet QKe; Fortebio, Menlo Park, CA, USA), which detects changes in mass (protein density) on a biosensor; changes in the reflected interference wave pattern between the sample and an internal reference layer result in a phase shift that can be followed in real time in both kinetic and quantitative modes [22].
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The assay has a diagnostic sensitivity and specificity of 97.7% and 100%, respectively, a lower detection limit of 50 viral gene copies/PCR, and can be adapted to either a qualitative or quantitative mode.
The molecular hydrophobicity was then extensively applied to diverse known aromatic selenium GPx mimics including diaryl diselenides and ebselen derivatives to explain their GPx-like activities in comparably quantitative mode, which could be helpful in designing new improved GPx mimic analogues in each series.
Data analysis was performed with Analyst 1.4.1 in the quantitative mode.
Then the interval of the quantitative mode is p, the signal value is quantized as 0, p, 2 p,…, np, where p = 2 t, n = ⌊ L / p ⌋ (where ⌊·⌋ is a floor function).
Distribution of rust severities in all environments indicated a quantitative mode of disease distribution with the Njoro main season (KenMain13) recording both higher severity and wider distribution of scores (Fig. 1a).
AFM experiments on yeasts immobilized on PDMS stamps were performed with a Nanowizard III form JPK JPK Instrumentss, Berlin, Germany) in contact mode, Quantitative Imaging mode (QI) [ 46] and Force Volume mode (FV).
The chapter presents quantitative observation modes, which produce direct maps of the sample magnetization configuration.
In addition, for blast charges of 400 and 800 g, the quantitative failure modes of crater, spall, and breach were not observed in NHC panels under contact explosions.
These nine associations described above could then be classified into three groups, namely, three quantitative trait modes (QTMs): three with additive (Figure 2A 2C), three with dominant (Figure 2D 2F) and three with overdominant effects (Figure 2G 2I).
Assignment of quantitative trait modes (QTMs) to each associated marker and linear regression models involving all significant markers for a given phenotype were described previously [23] with minor modifications.
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