Not only has the twin emphasis on quantitative methodology and vocational instruction failed to achieve genuine educational breakthroughs; but it has apparently had deleterious effects.
By quantitative guidelines we mean instruction for initial density, spacing, mixing proportions, thinning or regeneration cuts for mixed-species stands based on dendrometric characteristics (e.g. species-specific tree numbers, stand areas, SDI, basal area, or volume per unit area for the development of the remaining and removal stand).
We are grateful to Dr. Lei Mao for instruction in quantitative gel analysis and Dr. Karin Schmelz for advice on the FACS analysis.
The preceding descriptions illustrate two examples of how we can incorporate quantitative competencies into biology instruction.
One common theme throughout the literature is the need for increased instruction in quantitative biology (Brent 2004; Gross, 2004; Hoy, 2004).
The assay was performed according to the manufacturer's instructions and quantitative PCR reactions were run on an Applied Biosystems 7300 Real Time PCR machine.
Design Quantitative study of journals' instructions to authors (in June 2012) and qualitative study of editors' and publishers' views on trial registration and publication bias (carried out in Autumn 2012).
Cell migration (on collagen I or BSA) and invasion (Matrigel plug) assays were performed according to the manufacturer's instructions (collagen quantitative cell migration assay; Chemicon International , Inc.
One microgram of RNA extracted with TRIzol (Invitrogen) was converted into double-stranded cDNA with the cDNA synthesis kit ImProm™-II Reverse Transcription System (Promega), according to the manufacturer's instructions, using quantitative real-time PCR.
The concentration of TP protein in patient serum samples (sTP) was determined using a commercially available ELISA system (E90948Hu; USCN Life Science Inc., Wuhan, China) according to manufacturer's instructions for quantitative measurements.
