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Quantitative incorporation of the 6F monomer into the copolymer was monitored using FTIR and NMR analyses.
The quantitative incorporation of the initiator into the polymer chains was demonstrated by 1H NMR and MALDI-ToF MS measurements.
The quantitative incorporation of telechelic phosphine oxide functionality was confirmed using 1H NMR spectroscopy and elemental analysis.
The prediction was finally upgraded to a stochastic Monte Carlo simulation, which allowed quantitative incorporation of uncertainty, stemming from various sources.
This biosynthetic protein showed quantitative incorporation of the non-natural amino acid, as determined by mass spectrometry, and it revealed a unique emission spectrum due to the altered chemical structure of its fluorophore.
Following exhaustive dialysis, conversion of the anhydrides to esters was confirmed by IR spectroscopy and quantitative incorporation of the ethanolamine group was determined by H NMR (Fig. 2).
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Here we show that a combination of a carbodiimide and an organocatalyst induces near-quantitative incorporation of any of the four ribonucleotides.
Lowering the temperature to 0 °C, conditions close to those of eutectic ice phases, led to near-quantitative incorporation of UMP after 5 d in 0.5 m HEPES buffer.
Pre-activated nucleotides typically used for copying include imidazolides, 12 methylimidazolides, 13 and oxyazabenzotriazolides. 14 We recently showed that when the latter react with immobilized template primer duplexes, near-quantitative incorporation of any of the four nucleotides (A/C/G/U) is found.
TrIQ also does not require quantitative label incorporation, because the quencher (Trp) is always encoded in the protein (see Figure 1).
Therefore, combined with the inferences of this study that protein precipitation for NMR analysis offers reliable quantitative data, incorporation of serum/plasma protein precipitation for both NMR and MS detection opens avenues to exploit their combined strength in the metabolomics field.
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