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These results demonstrate that the microbial activity and hot-spot development can be monitored by using a non-invasive quantitative imaging system that allows real-time monitoring of spatial oxygen distribution.
Samples were blotted onto nitrocellulose membranes, probed with appropriate antibodies, and protein bands were identified by chemiluminescence and quantified using a Fujifilm LAS-3000 plus quantitative imaging system (FujiFilm, Valhalla, NY).
This step was followed by 1 cycle of 72°C for 7 min. The resulting PCR products were separated by gel electrophoresis through a 2% (w/v) agarose gel in Tris-acetate buffer, stained with ethidium bromide, and visualized under UV light with a Fluor-S MultiImager quantitative imaging system (Bio-Rad).
Using a novel, automated quantitative imaging system, VECTRA™, epithelial staining in both the nucleus and cytoplasm was quantified and compared against clinicopathologic variables.
Nitrocellulose membranes probed with monoclonal Abs against mouse AnxA1 and galectin-3 were revealed with Western Lightning Chemiluminescence Reagent Plus (ECL; Perkin-Elmer, Boston, MA) using the VersaDoc 3000 quantitative imaging system and Quantity One software (Bio-Rad).
DOI: http://dx.doi.org/10.7554/eLife.06259.010 10.7554/eLiFigure59.011 Figure 3 figure supplement 1. Overview of the high-throughput quantitative imaging system for single-cell fluorescent intensity measurements in C. elegans.
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pHi measurements were performed using a quantitative imaging microscopy system (ISee Imaging Systems, Raleigh, NC) as previously described [24].
The Vectra 3 is an automated, high-throughput quantitative pathology imaging system.
To determine the accuracy and repeatability of an automated quantitative fluoroscopic imaging system for measuring knee laxity.
Chemiluminescence was acquired with a quantitative digital imaging system (Quantity One, BioRad, Hercules, CA) allowing to check for saturation.
According to the protein concentration previously measured, equivalent volumes of supernatant and dissolved pellet were loaded to run Western blot and G-actin/F-actin ratio was quantified using a quantitative digital imaging system.
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