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Both qualitative and quantitative image analyses were performed.
The strut and pore sizes of the parts were measured by quantitative image analyses.
The quantitative image analyses revealed the presence and evolution of material stagnation zones in the deformation field, as well as the accumulation of deformation in the form of banded segments underneath the indenter that exhibited high incremental strains.
All quantitative image analyses were performed in blind conditions.
We subsequently focused on quantitative image analyses of the nucleus.
Therefore, we used p-Nephrin labeled with 15% or less Alexa488 for all quantitative image analyses.
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Semi-quantitative image analyses of the protein expression of BMPs, BMP receptors, total Smad 1/5/8 and pSmad 1/5/8 were performed using the Adobe Photoshop software (Adobe Systems Incorporated, CA, USA, version 10.0).
With this method, both semi-quantitative and quantitative digital image analyses have been proposed as tools to compare samples [ 1, 2].
An alternative technique to quantify angiogenesis is presented based on ISH of miRNA-126 in primary tumour tissue and using a specific LNA based probe and new quantitative systematic image analyses.
Based on the visual observations of key biological descriptors such as the density and shape of microvillis, we pursued the quantitative SEM image analyses by estimating cell surface roughness as an approximation to microvilli density/coverage, shape/geometry and length.
Described differences in preservation of chromocenters were confirmed by quantitative, automated computational image analyses of 3D confocal stacks (object counting, supplementary online material S 2).
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