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Accuracy and precision in quantitative fluorescence microscopy.
Waters, J. C. Accuracy and precision in quantitative fluorescence microscopy.
Schmied, J.J. et al. DNA origami based standards for quantitative fluorescence microscopy.
N.H. performed the gene expression, GCaMP6f analysis and quantitative fluorescence microscopy studies to identify the molecular basis.
We performed single-cell measurements using quantitative fluorescence microscopy and flow cytometry to monitor a target gene's protein expression in the presence and absence of regulation by miRNA.
Here, I focus on the parameters of digital image acquisition that affect the accuracy and precision of quantitative fluorescence microscopy measurements.
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To test the hypothesis that MyD88-NFκB signaling contributes to the ability of macrophages to clear Mp, we analyzed the survival of EYFP-Mp in cultures of WT and MyD88−/− BMM using both semi-quantitative fluorescence microscopy and real-time PCR.
The aim of this study was to evaluate the pharmacokinetics of meta tetrahydroxyphenyl chlorin (mTHPC) on different tissues of interest in a hamster tumour model and to confirm our earlier animal studies on semi-quantitative fluorescence microscopy.
Our earlier animal studies on semi-quantitative fluorescence microscopy (FM) (Andrejevic et al, 1996; Andrejevic Blant et al, 2000) have shown the much lower uptake and retention of mTHPC in striated muscle as compared to normal oral mucosa, squamous cell carcinoma (SCC) and smooth muscle.
Here we will first present characterization and validation steps required for quantitative Fluorescence Fluctuation Microscopy (FFM) experiments [6].
Using in vivo quantitative single-molecule fluorescence microscopy of kinesin II and OSM-3 motor dynamics in C. elegans cilia, Peterman and colleagues show that kinesin II loads cargo at the base, whereas OSM-3 transports the cargo to the tip.
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