It not only overcomes the limitations of other methods but also provides an absolute and quantitative expression measurement [ 16, 17].
Quantitative expression measurement of these two candidate biomarkers may potentially be used to eliminate the effect of differences in preanalytic processing of specimens by normalizing H-scores derived from test biomarkers of interest.
Quantitative gene expression measurements provide an explanation for the discrepancies observed for these samples.
The mean signal ratio of the two fluorescent intensities (Cy-5 cRNA test /Cy-3 pooled cRNA) is expressed as a logarithm (base 2), providing a relative quantitative gene expression measurement between two samples.
The mean signal ratio of the two fluorescent intensities (Cy-5 treated cRNA/Cy-3 reference cRNA) is expressed as a logarithm (base 2), providing a relative quantitative gene expression measurement between two samples.
We investigated the effect of 20 different combinations of 12 amino-acid substitutions within functional domains of the PIV-5 F glycoprotein, by performing cell surface expression measurements, quantitative fusion and syncytia assays.
Protein expression measurements by quantitative immunofluorescence and immunoblotting have been carried out on these minimal endothelial cell samples [29], [33], along with restricted, targeted gene expression analyses [34].
aEstrogen receptor positive, ≥ 20 fmol/mg protein (enzyme immunoassay) for the Stiftung Tumorbank Basel data set; for The Netherlands Cancer Institute study, see [5,6] Gene expression measurements by quantitative RT-PCR were performed as reported previously [ 24].
We used high-resolution, quantitative mass spectrometry to collect protein expression measurements from human, chimpanzee, and rhesus macaque lymphoblastoid cell lines and compared them to transcript expression data from the same samples.
Color Measurement is the quantitative expression of color and as indicated previously, there are a number of methods to quantify color.
