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To confirm our findings, quantitative epifluorescence microscopy images of labelled cells were obtained.
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In recent years, microorganisms have been primarily quantified directly from the environment using techniques such as quantitative PCR (qPCR), epifluorescence microscopy, and flow cytometry.
Rab23 knockdown significantly reduced ciliary targeting of SSTR3, as assessed by epifluorescence microscopy and both quantitative metrics.
GRAF1-depleted cells were assessed for their ability to endocytose dextran (allowing assessment of total endocytic capacity), both by epifluorescence microscopy and by a quantitative fluorimetric assay.
No obvious localization or quantitative differences among clones were observed under epifluorescence microscopy, and the size of the expressed proteins was comparable as predicted (Fig 3C), excluding truncation artifacts.
E12-positive cells were observable in whole embryos for an additional 48 hours of development by epifluorescence microscopy, although confocal microscopy or sectioning the embryos is required for quantitative analyses.
The relative abundance of methanogens was monitored with epifluorescence microscopy.
Transverse sections of leaves were subjected to the epifluorescence microscopy.
Epifluorescence microscopy analyses and Live/Dead tests showed that all films are toxic for Euglena gracilis.
Bacterial adhesion on nitrided titanium alloy was analysed by both epifluorescence microscopy and scanning electron microscopy.
Bacterial communities associated with biofilms were determined using epifluorescence microscopy and high-throughput sequencing.
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