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She later applied the discovery of the technique, quantitative electron microscopic radiography, to biological questions related to synapses.
The numerical density of synapses and mean synaptic height were determined using design-based quantitative electron microscopic techniques.
Our light- and quantitative electron microscopic observations provided support for the view that KCC2 protein is developmentally regulated in postnatal rat brain.
In the latter situation detailed quantitative electron microscopic studies may be necessary to show differences in the G-ratio in affected areas.
In the present study, we have applied a highly sensitive, high-resolution quantitative electron microscopic immunogold technique to investigate the densities of the Kv4.2 subunit in 13 distinct axo-somato-dendritic compartments of CA1 PCs.
Our approach was to perform in vivo juxtacellular labelling of MSNs in the D1 and D2 BAC transgenic mice together with immunolabelling for VGluT1 and VGluT2 and quantitative electron microscopic analyses.
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Since stress is also known to alter the expression of leu-enkephalin in various brain regions, this study was designed to determine whether acute or chronic stress had an effect on mossy fiber leu-enkephalin levels in females or males, through the application of correlated quantitative light and electron microscopic immunocytochemistry.
Another limitation of this study might be the lack of quantitative data from electron microscopic findings.
For electron microscopic analysis, a Hitachi H-7000 electron microscope (Hitachi, Japan) was used.
Scanning electron microscopic (SEM) measurements were performed on a Philips XL30 electron microscope (Philips, Amsterdam, The Netherlands).
Electron microscopic examination was conducted by using a Tecnai 10 electron microscope (Philips, Amsterdam, the Netherlands).
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