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The known limit of qPCR-HRM is the difficulty in designing primers efficient for both qualitative detection and quantitative dosage.
Large rearrangements were identified using quantitative dosage analysis of the data obtained from NGS.
The quantitative dosage of BNP was done by the immunofluorescence method (Biosite Triage BNP test).
We therefore believe that the effect of these ATP motif mutants is an end result of a balance between quantitative (dosage effect) and qualititative (e.g. mislocalization, phosphatase affected, etc).
This analysis showed a continuous decrease in CIN when NPL3 copy number increased from 1 to 3 copies in the genome, but further increases began to reverse the CIN suppression phenotype, demonstrating quantitative dosage effect of NPL3 on CIN.
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Feeny [ 17] suggested that plant defense strategies should differ depending on their "apparency"; plants that are long-lived and/or physically large will always be found by attacking insects and should possess constitutive chemical defenses such as tannins, terpenes, and flavanoids (see also [ 18- 21]) that have a quantitative, dosage-dependent effect.
In that case, the vector x = (x1, x2 T includes two quantitative factors: dosage of NPs x1 and post-exposure time x2.
Somerville et al. (2005) and Berg et al. (2007) noted that the contrasting effects of deletions versus duplications for the Williams syndrome region provide evidence for strong effects of quantitative gene dosage variation on human language, sociality, and visual spatial abilities.
The effects of quantitative metabolites are dosage dependent and the higher these chemicals' proportion in the herbivore's diet, the less nutrition the herbivore can gain from ingesting plant tissues.
This strategy, called qPCR-HRM, is based on a real time PCR assay (gene dosage quantitative analysis) combined with HRM curves (qualitative analysis of punctual mutations) in a single tube [7].
Thus far, most genome-wide analyses have dichotomized their dependent variable into cases and controls, which may result in a significant loss of power and particularly for phenotypes related to drug dosage quantitative trait analyses are needed.
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