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In order to obtain quantitative data, we implemented an automatic segmentation of islets of Langerhans in xfOCM tomograms based on an active contours algorithm.
Most importantly, using the quantitative data available from tracking and segmentations of complete connectomes, many biological questions can be generated, such as: (i) the relationship between the number of branches and the length of each segment in a population of axons; and (ii) the spatial distribution of motor neurons or the optimal layout of the motor neurons in a population of axons.
Segmentation and extraction of quantitative data from OCT images are challenging [ 23– 25] but are required to facilitate and improve diagnosis.
Our pipeline and novel segmentation procedure may benefit end-users of SR microscopy to analyze their images and extract biologically significant quantitative data about them in user-friendly and fully-automated settings.
Very little quantitative data.
Quantitative data are shown in e.
Quantitative data are shown in i.
Quantitative data were depicted in g.
Quantitative data are means ± s.e.m.m
Quantitative data are depicted in b.
Quantitative data are depicted in e.
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