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HPLC revealed quantitative complexation.
Quantitative complexation of 177Lu III) with DOTAZOL was obtained within 30 min at 98 °C.
Quantitative complexation at room temperature occurred within a few minutes without forming any side products.
At the pH-optimum, almost quantitative complexation was reached after 30 min at room temperature.
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Quantitative 68Ga-complexation was achieved using 3 nmol of the respective DOTA- or DOTAGA-conjugated PSMA inhibitors (95°C, 5 min, pH adjusted to 3.5 and 4.5 by addition of 2.7 M HEPES, respectively), allowing their use in in vitro studies without further purification.
As with the cold complex, the complexation proceeds instantaneously and quantitatively, and no heating is required.
Through analyzing the change of fluorescence intensity of protein chromophores caused by complexation, a quantitative mathematical equation has been established and the average number of bound proteins per neutral polymer (nb) can be calculated accurately without destroying the dynamic equilibrium of aqueous complex system.
We tested our minimalist design strategy in the native cellular environment of yeast, and compared these in vivo results with in vitro quantitative measurements of protein:DNA complexation.
Complexation occurred fast and quantitative (RCY > 99%%).
This study aims at developing a quantitative structure property relationship (QSPR) model for predicting complexation with β-cyclodextrins (β-CD) based on a large variety of organic compounds.
Formulation of a stable lipid membrane with good drug complexation characteristics is a complex experimental problem as there are many possible components (qualitative variables) with a wide range of relative amounts (quantitative variables) in the recipe.
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