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The hybridization of fluorescently-labelled unamplified ChIP DNAs, coupled with a highly sensitive and quantitative array platform [19], allowed us to identify reproducibly (Figure S1), and validate with real-time PCR (Figure S2), a wide range of regulatory features across the SCL locus as described below.
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Systems, such as the Fluidigm quantitative PCR array described here, can provide powerful assessments of the effects of diet, environment, and management on physiological pathways affecting production parameters.
Thus, when the expression of 84 angiogenesis-related genes was investigated with a quantitative PCR array, 25% of these genes showed more than a 10-fold difference in expression.
Both the quantitative glycan array binding and human tissue binding results of Alb58 HA show substantial avian receptor binding.
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A mouse oxidative quantitative PCR array was purchased (QIAGEN, Valencia, CA, USA) and analyzed according to manufacturer's instructions.
Quantitative PCR array on select genes revealed that the two compounds have different and often opposite effects on angiogenic gene expression.
Other problems that are obviously to all gene expression assays (irrespective of whether quantitative PCR, array technology or RNA sequencing is used) are intratumoral and intercellular heterogeneity.
The Mouse Alzheimer's Disease real-time quantitative PCR array (PAMM-057Z Qiagen) used in our study contains 84 unique genes related to Alzheimer's disease.
However, quantitative expression array analysis of clinical cancer samples with high stromal content is challenging since the ratio of epithelial tumor cells to stromal cells can vary greatly.
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