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A generalized analysis system was developed applying a unified data format to quantitation results from a wide range of omics data, across a collection of experiments for a target organism.
Comparison of the real-time PCR quantitation results from 35 clinical serum samples with those obtained by COBAS Amplicor HBV DNA monitor kit (Roche Diagnostics) revealed a significant correlation (r = 0.92) for all the samples.
Relative quantitation results were normalized with A. gambiae ribosomal protein S7 as internal standard and analyzed by the 2 ΔΔCt method [27].
The amplification signal obtained with a primer pair flanking the psoralen adduct was 49% (input plasmid) and 6% (eluted plasmid) of that obtained with primers flanking a control region, consistent with the gel quantitation results.
The quantitation results for all of the detected peptides are shown in Supplementary Table 2.
For quantitation results, peptides with swath_score ≥ 0.99 were kept for further analysis.
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Real-time PCR quantitation result revealed that after 24 h treatments A. parviflora showed highest antiviral activity, followed by A. bracteosa, while B. lycium extract had low (35%) and C. lemon has no antiviral effects.
The quantitation result (peptide ion volumes in thompson·sec) for each peptide was presented in a diagram.
Internal standard quantitation resulted in increased precision with similar accuracy to standard addition for most OPAHs using 2-fluoro-fluorenone-C as an internal standard.
Point-spread function (PSF) or PSF + time-of-flight (TOF) reconstruction may improve lesion detection in oncologic PET, but can alter quantitation resulting in variable standardized uptake values (SUVs) between different PET systems.
Data were analysed using the comparative CT method for the relative quantitation of results.
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