Indexed mRNA-seq libraries were generated from 1 μg total RNA following the Illumina TruSeq standard unstranded protocol and then checked for quality and quantitated with the Agilent Bioanalyzer and the Kapa Biosystems qPCR library quantitation method.
DapG2 gene primers and probe (Additional file 1: Table S2) were used to quantitate genomic DNA using reference B. anthracis DNA using the absolute quantitation method (Life Technologies, Carlsbad, CA).
A reliable and very sensitive quantitation method was finally obtained using this integrated microfluidic technology.
The DNA quantitation method is utilized twice during the extraction procedure.
The development of this simple and rapid CAV-2 quantitation method should be helpful for process development and monitoring.
The developed quantitation method was fully validated for its recovery, matrix effect, linearity, repeatability, precision and accuracy, and storage stability.
Fijalkowski et al. (2014) detected the presence of E. coli O157 H7 in wastewater and sewage sludge in Poland by quantitation method of (EMA) real time-PCR.
We therefore performed chest dynamic planar imaging, instead of arterial blood sampling, to estimate the input function and establish noninvasive quantitation method of rat CBF using the image-derived input function.
Data were analyzed by relative quantitation method using standard curve.
The comparative quantitation method (ΔΔCt) was used to contrast the different conditions [50].
Quantitation of target RNA, normalized with an endogenous control (Cyclophilin), was performed using the absolute quantitation method (Applied Biosystems).
