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The quantitative determination of the lignans, phyllanthin and hypophyllanthin, their precision, specificity, quantitation limit and detection limit have been reported in our previous study [ 28].
Quantitation limit.
Method validation parameters included linearity, recovery, repeatability, precision, stability and quantitation limit.
The following validation characteristics were addressed: specificity, detection limit, quantitation limit, linearity, precision, accuracy and robustness.
The detection limit (DL) and quantitation limit (QL) were 0.09 and 0.29 μg mL−1, respectively.
The lower quantitation limit was estimated to 980 copies of RNA/reaction.
The assay has a lower quantitation limit generally below 0.04%, depending on the cytokine subset.
Whole method produces quantitation limit for indapamide of 1 ng/ml.
A quantitation limit of 0.2 mg L-1 was reported for both Cordyceps sinensis and Ganoderma lucidum.
The method was validated in terms of linearity and range, accuracy, precision, specificity, robustness, detection limit, and quantitation limit.
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The lowest quantitation limits of these techniques were 10, 0.2, and 0.3 ng/mL, respectively.
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