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Although the concentrated rinse method does not detect the localized site of infection, it enables quantitation of other microbes in addition to Candida species [ 5].
To study changes of bone matrix global phosphorylation, we developed a strategy that combines a procedure for in vitro phosphorylation/dephosphorylation of fully mineralized bone in addition to quantitation of the global phosphorylation levels of bone matrix proteins.
Using liquid chromatography tandem mass spectroscopy (LC MS/MS), in addition to quantitation, the d-SSwitch approach allows identification of each individual modification site in the cysteome.
In addition to quantitation, the lymphocyte assays allow molecular analyses of in vivo mutations, the definition of background and induced mutational spectra, and the search for unique changes for characterizing specific mutagens.
In addition to absolute quantitation, samples were also evaluated for the melting temperature (Tm) of amplified products using LightCycler Software V 4.1.
In addition to careful quantitation of input genomic DNA, q-PCR could be used following digestion and ligation of bar-coded adapters to normalize template numbers derived from different genotypes at the template pooling stage [ 35].
In addition to the better quantitation with BSAS, there were multiple benefits of using this method.
In addition to measuring α-HB by absolute quantitation, targeted assays were also developed for candidate IR biomarkers identified by RF and correlation analyses, with examples of representative biochemical classes highlighted in Figure 5.
In addition to rigorous identification, the quality of the automated Metabolyzer integration (basis of quantitation) was verified for each biochemical.
In addition to minimizing the amount of RNA needed for analysis, the miniaturization allows quantitation of expression of many genes in hundreds of samples on a single slide.
In addition to every CpG site not being statistically significant, the variation in methylation quantitation, shown by standard deviation, was much greater in the Sanger/ESME data with standard deviation ranging from 5%to30%0%.
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