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To avoid possible interference from naturally occurring endogenous acids and quantitation errors 3- 4-chlorophenyl) propionic acid was chosen as an internal standard.
Fewer than 4% of the data points were found to have peak matching and quantitation errors of >10%, see legend to Table 1).
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The 'traffic light' assay also demonstrated a high degree of analyte detection when testing spiked milk samples (92 101%) and accuracy (quantitation error <8% of the mean).
To determine the quantitation error of phosphopeptides, DNP-regulated protein expression changes were used to determine the median H/L ratio.
Differences in sample read counts are more likely due to pipetting or quantitation error when the sequencing libraries are initially combined prior to the enrichment process.
The increased accuracy in the BSAS method was attributed to the decrease in quantitation error over the traditional approach, seen in the standard curves generated.
Although the coincident measurement of a pair of annihilation photons by a ring of detectors surrounding the patient is disturbed by random and scattered (Fig. 2) coincidences as well as by events lost due to tissue attenuation, the related errors can be corrected sufficiently so that the final quantitation error is in the range of a few per cent.
We have described here two possible sources of error that might hinder the ability to pool samples effectively: inaccurate DNA quantitation, and sampling error in tissue collection.
The generation of peak list, SILAC- and extracted ion current-based quantitation, calculated posterior error probability, and false discovery rate based on search engine results, peptide to protein group assembly, and data filtration and presentation was carried out using MaxQuant.
Major points: 1) There is a general concern throughout the manuscript that interpretations about relative potencies (and half-max ligand concentrations) are made without quantitation or (therefore) error bars – and it is not stated how many repeats of each analysis were done.
Therefore, the inconsistencies between dye-labeled assays are likely due to labeling efficiency or errors during quantitation of the dye signal.
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