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A rapid and sensitive SYBR Green I-based real-time PCR assay was developed and used for the detection and quantitation of orthopoxvirus by using ECTV in this study.
Absolute quantitation was enabled by using external and internal standardization (see Material and Methods).
Primers for RT-qPCR were purchased from SuperArray for DHS quantitation or designed by using Primer 3 software.
Image acquisition and quantitation was done by using Volocity software.
Toxin B quantitation was performed by using a Vero cell cytotoxicity assay (11 ).
Quantitation is achieved by using the intensities of the top three peptides (MS1).
Seventeen Z stacking images with 0.2 μm intervals were acquired and quantitation was done by using Volocity software.
Since KL-6 has not been evaluated in COPD lungs earlier, the distribution and quantitation was conducted by using digital image analysis.
Quantitation was achieved by using PDA detection at 240 nm for compounds 1 and 3– 5 and 277 nm for compound 2 based on retention time and UV spectra compared with those of the standards.
For counts of each of the species present in the multispecies oral biofilm, conditions were standardized for quantitation by qPCR using pure cultures of the five species.
Quantitation of diltiazem is performed by using an analyte to internal standard ratio and a concentration-weighted linear regression.
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