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Overall, the digital quantitation of BSAS improved the quantitation and statistical power over the Sanger method, which has an analog output with CpG methylation quantitation being a function of the area under the curves for the C and T traces.
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Quantitation is not currently available.
Relative quantitation was performed using the Applied Biosystems software.
Relative quantitation was performed using the comparative CT (∆∆CT) method53.
Sample quantitation was performed using Invitrogen's Picogreen assay.
A method for its quantitation is proposed.
Quantitation was accomplished with internal standard method.
All other DNA quantitation was performed using Pico-Green fluorescent dye (ThermoFisher, MA, USA).
All GFP-LC3 puncta quantitation was performed by observers blinded to experimental conditions.
Image treatment and quantitation were performed using the software EZ-C1 3.20 (Nikon).
Adenine quantitation is required for a variety of applications.
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