Sentence examples for quantitated with the from inspiring English sources
RNA was quantitated with the RiboGreen RNA quantitation kit (Molecular Probes, Eugene, OR, USA), dissolved in diethylpyrocarbonate-treated H2O, and stored at -80°C until use.
Indexed mRNA-seq libraries were generated from 1 μg total RNA following the Illumina TruSeq standard unstranded protocol and then checked for quality and quantitated with the Agilent Bioanalyzer and the Kapa Biosystems qPCR library quantitation method.
The average fragment size was estimated by Bioanalyzer (Agilent) and libraries were quantitated with the qPCR-based Library Quantification Kit (KAPA Biosystems).
All 15 samples were individually barcoded and quantitated with the KAPA Library Quantitation Kit for Illumina Platforms (Kapa Biosystems, Wilmington, MA) in conjunction with an Agilent Bioanalyzer DNA 1000 analysis (Agilent Technologies, Santa Clara, CA) for fragment size determination.
The membranes were developed using the SuperSignal West Femto Chemiluminescent Substrate (Pierce , Thermo Scientific Pittsburgh, PA, USA) and were visualized and quantitated with the Bio Spectrum 500 Imaging System (UVP, Cambridge, UK).
The LOD and LOQ were estimated by analysis at several concentrations of analytes, diluted appropriately to estimate the lowest possible concentration that can be detected and quantitated with the optimized experimental conditions.
Only peaks with the correct combination of m/z transition and RT were considered to be positively identified as the analyte of interest and were subsequently quantitated with reference to the standard curve of the particular analyte.
The DNA was then quantitated with a spectrophotometer, and the concentration diluted to 2.5 ng/µL.
The PCR products were quantitated with Sybr green (Invitrogen) and the copy number was calculated by comparison with a standard curve of cloned MK3 template, serially diluted in control spleen DNA and amplified in parallel.
DNA was quantitated with PICO green and the same amount of DNA was used for each PCR reaction.
Labeled bands were detected by Luminata Crescendo Western HRP substrate (Millipore, Billerica, MA, USA) and images were captured and the intensity of the bands was quantitated with ImageReader LAS-4000 (Fujifilm CorporaTokyo, Japan Japan).
