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The expression level of proteins was quantitated with respect to β-actin.
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Moreover, roughly 1,900 of these proteins were quantitated with at least 2 unique peptides.
In addition, the efficiency of coreceptor use can be accurately quantitated with flow cytometric analysis.
Band density was quantitated with ImageJ software.
PCR products were quantitated with a gene-specific probe.
CER was quantitated with the DGK assay [46].
Gels were scanned with a STORM Phosphorimager (Amersham) and label quantitated with Image Quant software (Amersham).
The DNA was then quantitated with a spectrophotometer, and the concentration diluted to 2.5 ng/µL.
The radioactivity retained on filters was quantitated with a scintillation counter (Wallac 1409).
Blots were quantitated with the imaging and analysis software ImageJ 1.42d (http://rsbweb.nih.gov/ij/).
Luminescence and fluorescence were quantitated with a Mithras LB 940 apparatus (Berthold Technologies, Oak Ridge, TN, USA).
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