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Figure 4 displays the average quantitated results of comparable OPAHs in all four matrices from each chromatographic system.
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External and internal calibrations with known amounts of NAD+ were used to quantitate results.
Manual interpretation of data using publically available databases such as KEGG pathways and the LipidMAPS databases may help place quantitated lipidomics results into a meaningful biological context.
The degree of reduction varied somewhat between the different siRNAs, but these siRNAs were capable of reducing protein levels by 60 95% (quantitated blots, results not shown).
We further quantitated these results using high content image analysis which showed that cleaved caspase-3 positive cells increased with increasing radiation doses.
To verify and quantitate these results, we next performed qPCR on cDNA from the five CF2 mutants and a wild type control, using primers for Act88F, and the flight muscle specific isoforms of mhc and Tn I.
To quantitate the results, the batch 2 sera were tested three times, each time in batches of 10 controls and 10 infected; to normalise the results for different blots, the 70 kDa signal strengths were calculated relative to the average signal for all 20 sera in the experiment.
To quantitate these results, we measured migration rate and directional persistence.
To quantitate the results from Figures 2 through 5, MCs were counted under the microscope (six mice per experimental group and 10 fields per section per organ), and the mean numbers reflected in the graph.
It may be important to repeat the experiment and try and quantitate the results in some way to substantiate the argument that this ZFN pair is safer when delivered by LP.
While we saw some reduction in gp120 binding, it was difficult to quantitate the results due to the intra-assay differences in gp120 binding in the absence of antibody.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com