Exact(1)
The OD values were quantitated into the cell viability percentage by the equation: cell viability % = (OD value of experimental group / OD value of control group) × 100%%.
Similar(59)
Total RNA was isolated from porcine muscles and C2C12 cells using the RNeasy Mini kit (Qiagen), quantitated in an Agilent 2100 bioanalyzer and converted into cDNA with random primers using a Reverse Transcription System (Promega).
The concentration of neuroimmune SP increases in the brain, leaking into the bloodstream, where it can be quantitated in serum or plasma samples.
At the end of the incubation time, the cells that invaded into the lower chamber were detached, lysed, stained with CyQuant and quantitated in a fluorescent plate reader.
Migration rate is quantitated in the right panel.
Control plasmids that contain approximately 1000 bases of human genomic sequence with a specific mutation of interest positioned near the middle of the insert and a nearby 6-bp molecular barcode were synthesized, linearized, quantitated, and spiked into genomic DNA derived from formalin-fixed, paraffin-embedded prepared haparaffin-embedded prepared copy number ratios.
Manual interpretation of data using publically available databases such as KEGG pathways and the LipidMAPS databases may help place quantitated lipidomics results into a meaningful biological context.
The design was adapted from a method that utilizes methyl-sensitive restriction enzymes [ 14], which when applied to genomic DNA would produce a set of digested fragments that could be quantitated and translated into an amount of DNA methylation present in the genome.
Cell survival after heating at 44°C for 0, 15, 30, 60, 90 or 120 min was quantitated by plating cells into 25 cm flask containing the medium.
Immunoprecipitates were subjected to SDS PAGE, and radioactivity incorporated into 6Myc-PAGE4 was quantitated with an X-ray film.
BrdU incorporation into DNA was quantitated by ELISA assay (Roche Applied Science, Indianapolis, IN) according to the manufacturer's protocol.
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