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Incorporated BrdU and pH 3 positive cells in each animal were quantitated in the same way except that a fuzziness setting of 64 was used.
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In an attempt to obtain preliminary information on quercetin levels, quercetin was quantitated in the plasma.
The secondary structure content was quantitated in the 200 240 nm range using K2D3.
All other cytokines and chemokines were measured at the Roswell Park Cancer Institute core facility flow cytometry laboratory using the multiplexed flow cytometry assay in which several cytokines can be quantitated simultaneously on the same sample.
To verify whether the increased mRNA levels were accompanied by alterations in protein levels, GluD1 protein was quantitated in extracts from mature neurons differentiated with the same protocol.
The supernatant was quantitated in a mass spectrophotometer by the protocol described by Wright and Upadhyaya (1998).
Toceranib was quantitated in plasma samples using the method previously described [ 7, 8].
The cDNA was quantitated in duplicate using SYBR Green Master Mix Applied Biosystemss, Foster City, CA, USA) as reported.
The incidence of VF was quantitated in all rats.
Organochlorine pesticides were quantitated by gas chromatography-mass spectrometry in the same manner as for PCBs, except that the column was a BPX-25 fused silica capillary column, 0.22 mm i.d.
BDE-209 was one of the least abundant congeners, but still quantitated in all pools.
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