Sentence examples for quantitated by using from inspiring English sources

The RNA was quantitated by using a RiboGreen RNA quantitation kit (Molecular Probes Inc., now part of Invitrogen Corporation, Carlsbad, CA, USA), dissolved in diethylpyrocarbonate-treated H2O, and stored at -80°C until use.

The amplified RNA (cRNA) generated in this manner was quantitated by using a NanoDrop® ND-1000 UV-Vis Spectrophotometer (NanoDrop Technologies, Wilmington, DE).

Chitinase activity was quantitated by using colloidal chitin as substrate, the assay mixture includes 1 ml colloidal chitin (10 mg/ml), 0.5 ml 50 mM acetate buffer (pH 5.0) and 1 ml enzyme.

Cleavage fractions were quantitated by using the Storm 820 phosphorimager along with the Molecular Dynamics software.

In rest experiments, the intensity of Western blot signals was quantitated by using an Odyssey Infrared Imaging System (LI-COR Bioscience, Lincoln, Nebraska).

The total number of Brn3a-positive cells in the retinas, located approximately at the same distance from the optic disk (7200 sq. microns, 40× magnification) was quantitated by using Scion Image analysis software (Scion Corp., Frederick, MD).

Developed films were imaged using a digital scanner (Canon), and blot intensities were quantitated by densitometry using the ImageJ software (NIH).

Results from three independent experiments were analyzed by Western blot, and the intensity of the bands was quantitated by densitometry, using AlphaInnotech FluorChem® HD2 instrument (Alpha Innotech, San Leandro, CA, USA) and analyzed using alphaeasefc software, provided by the manufacturer.

To assess the unique identity of the color codes, the fluorescence intensity of each microbead was quantitated by ImageJ using Eq. 1.

The cells were incubated at 37°C for 2.5 h, and then the concentrations of compound in the apical and basolateral side were quantitated by LC/MS/MS using standard analytical methods.

RNA was quantitated by O.D.260/280 using spectrophotometry DU640 (Beckman).