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All LCM-captured samples yielded sufficient total RNA (10 – 20 μg) that could be quantitated by the low-range Ribogreen RNA Quantitation Kit (Molecular Probes).
All samples were quantitated by the comparative cycle threshold C t) method for relative quantitation of gene expression, normalized to either GAPDH or β-actin [ 35].
Phosphate is quantitated by the color-generating phosphorylysis of a particular purine derivate.
In addition, utilizing probe S4, cyanide ion in real industrial samples can be rapidly recognized and quantitated by the naked eye.
In addition, the malondiadehyde (MDA) was quantitated by the thiobarbituric acid procedure and the total antioxidative capability (T-AOC) by a colorimetric method.
There is a positive correlation between the titers of C-strain vaccines titrated in rabbits and RNA copies quantitated by the multiplex real-time RT-PCR.
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The two-dimensional movement of the cells was quantitated by measuring the surface area occupied by the migrated cells using the Scion Image 1.62 software [ 37, 38].
The strength of the stalling was quantitated by measuring the amount of radioactivity in the bulge, relative to the intensity of the arc (Pelletier et al. 2003).
Migration was quantitated by measuring the width of the cell free zone at the time of the scratch (0 hours) and 24 hours after the scratch.
Neovascularization around the disk was quantitated by determining the number of angiogenic vessels within the CAM around the disk.
The level of PCNA in the cells was quantitated by measuring the absorbance at 405 nm, according to the manufacturer's protocol.
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