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Cytotoxicity and apoptosis were quantitated by means of (4,5-dimethylthiazo-2-yl -2,5-diphenyl tetrazolium bromide and terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling-based assays, respectively.
In these experiments cell mass was quantitated by means of crystal violet dye retention assay [ 21].
Adherent cell number was quantitated by means of CyQUANT Cell Proliferation Assay Kit (Molecular Probes) according to the manufacturer's instruction.
The sera C3, C4, and CRP levels were quantitated by means of immunonephelometry on the Behring nephelometer systems (BN II).
Tau bound and unbound to microtubules was separated by centrifugation and the levels were quantitated by means of SDS-PAGE and CBB staining.
Urease B enzymatic activity was measured and the inhibitory effects of U001 and U002 were quantitated by means of an inhibition assay.
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Nine intense sweeteners (ACS-K, ASP, ALI, CYC, SAC, NEO, NHDC, SCL and DUL) were successfully separated and quantitated in various food products by means of HPLC ELSD [ 21].
Also, the development is described of a system to detect and quantitate DNA adducts at the single-cell level by means of immunofluorescence microscopy, which allows the analysis of small samples of human tissue with preservation of cell morphology.
Thus, the target protein production could be easily quantitated by indirect determination of the fluorescence intensity of mCherry in crude E. coli culture in real-time.
Telomerase activity was measured by TRAP and the levels of telomerase activity were quantitated by scanning of the autoradiographs with densitometry.
The resulting LTA was quantitated by measurement of phosphorus [ 13].
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