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A constant amount of the RNAs was quantitated based on the standard curve.
The product was quantitated based on the standard curve of GlcA-β-MU.
The concentration of PCR DNA was quantitated based on the band intensity relative to a DNA standard using the software Image Lab (Bio-Rad, Hercules, CA).
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The PCR data was quantitated based on a standard curve generated using fourfold serial dilutions of the target genes.
Curcuminoids were quantitated based on standard curves for curcumin (analytical grade) by integration of the peaks with the FPLC Unicorn™ (version 5.10) program (GE Healthcare Life Sciences, Piscataway, NJ, USA).
Post normalization, protein bands were relatively quantitated based on densitometry values and are listed in Table S1 (also see Table S2 for the densitometric values for the identified bands reported in Table S1).
Enriched samples were quantitated based on Quant-iT dsDNA HS Kit (Invitrogen) and qPCR.
Results were reported only if they met qualitative GC/MS criteria (retention time, mass spectrometric ion-abundance ratios, and mass spectra) before being quantitated based on a 5−8 point calibration curve.
Expression of this glycoprotein antigen was quantitated by immunofluorescence flow cytometry based on the monoclonal antibody 9.2.27.
Further, most quantitated Tregs based on CD25 expression without the use of a reference marker by which to set the CD25 gate.
Thymidylate synthase expression was quantitated using a visual grading system based on the intensity of staining and was classified into groups from 0 to 3, where 0 and 1 were defined as low intensity, and 2 and 3 were defined as high intensity staining.
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