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A constant amount of the RNAs was quantitated based on the standard curve.
Post normalization, protein bands were relatively quantitated based on densitometry values and are listed in Table S1 (also see Table S2 for the densitometric values for the identified bands reported in Table S1).
The product was quantitated based on the standard curve of GlcA-β-MU.
Enriched samples were quantitated based on Quant-iT dsDNA HS Kit (Invitrogen) and qPCR.
The PCR data was quantitated based on a standard curve generated using fourfold serial dilutions of the target genes.
The concentration of PCR DNA was quantitated based on the band intensity relative to a DNA standard using the software Image Lab (Bio-Rad, Hercules, CA).
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Further, most quantitated Tregs based on CD25 expression without the use of a reference marker by which to set the CD25 gate.
The current analysis also shows that quantitating ELISAs based on measurements made in the interfacial region between two assay segments is prone to error.
Since the signal was too low to be detected by Nothern blotting, liver UCP-2 mRNA was quantitated using RT PCR based on a previously described method (Taylor et al, 1998).
Dietary exposure assessments of all drugs based on quantitated residue levels are within the Joint FAO/WHO Expert Committee on Food Additives safe regulatory exposure limits.
MTT (Sigma-Aldrich, St . Louis MO, USA) assay was used to evaluate the proliferation of SCAPs based on quantitating the insoluble purple formazan through colorimetric assay, which comes from mitochondrial succinic dehydrogenase.
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