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Drug concentrations in the skin were quantitated at the end of the diffusion experiment.
Nitrite was quantitated at the end of each experiment using the Griess assay.
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DNA samples from this replication cohort were quantitated at the National Genotyping Center (CeGen, Madrid) using the PicoGreen assay.
Genomic sequences were quantified based RepeatMasker matches to the TIGR Solanum Repeat Database v3.3 sequences and quantitated at the sub-class level [ 70].
To identify and quantitate surface lung metastatic nodules, at the end of the experiment, animals were euthanized and the chest cavity and trachea was exposed through a midline chest incision.
The cleavage of the peptide by the caspase releases the chromophore pNA, which can be quantitated at 405 nm.
The fluorescence signal was quantitated at 485 nm excitation and 538 nm emission by a microplate fluorometer (Fluoroskan Ascent, Thermo).
The enzymatic activities were quantitated at tested pH value at 20°C with ONPGal or PNPGlc.
The resultant absorbance of samples was quantitated at 450 nm using multimode plate reader (infinite M200, TECAN, Austria), and background was subtracted.
The number of apoptotic cells was quantitated at 24 hour intervals following head and neck irradiation.
After solubilization in DMSO, the mitochondrial reduction of MTT to formazan was quantitated at 490 nm (iMarkTM microplate reader, BioRad, Italy).
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