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Drug concentrations in the skin were quantitated at the end of the diffusion experiment.
DNA samples from this replication cohort were quantitated at the National Genotyping Center (CeGen, Madrid) using the PicoGreen assay.
The amount of the area of either green or red fluorescence of the organelles was quantitated at the pixel level using the Compix software to measure the size of the fluorescent area.
Nitrite was quantitated at the end of each experiment using the Griess assay.
Genomic sequences were quantified based RepeatMasker matches to the TIGR Solanum Repeat Database v3.3 sequences and quantitated at the sub-class level [ 70].
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Calibration check standards were run before and after each set of samples, and were considered successful if native OPAHs quantitated at ±30%% the true value for 90%% of compounds in each method.
DNA samples from this replication cohort were quantitated at TGen using the PicoGreen assay and individually genotyped for the selected SNPs by means of the TaqMan allelic discrimination assay.
Since the accumulation was quantitated at already-formed invadopodia, the decreases in protease accumulation for both integrin- and ILK-inhibited cells were independent of any effects on invadopodium formation or lifetime.
The mjA' inclusion bodies were automatically solubilised in the presence of 8 M urea and quantitated at 562 nm with the bicinchoninic acid assay.
The fixed ratio design of the cytotoxicity experiments allowed the concentration increments to be quantitated at 1/8, 1/4, 1/2 and 1.
The cleavage of the peptide by the caspase releases the chromophore pNA, which can be quantitated at 405 nm.
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