Exact(12)
Levels of TNF-alpha, IL1-beta, IL2, IL6, IL8, IL10, and IFN-gamma in synovial fluid were quantitated according to the manufacturer's protocol.
Glomerulonephitis was quantitated according to Berden scores [42].
Levels of CD8+/CD38+ T-cells were quantitated according to a previously published method [25].
Tris-Acetate 3 8% gels were run, Western blotted, visualized and quantitated according to manufacturer's instructions for the NuPage system (Invitrogen) and Odyssey Infrared imaging system (Licor Biosciences).
DNA fragmentation was quantitated according to Bertrand et al (1991).
ER and PgR immunohistochemistry were quantitated according to Allred score [ 10].
Similar(48)
TaqMan® MicroRNA Assays were used to quantitate miRs according to manifacturer's instructions (Applied Biosystems, Foster City, CA).
Stem-loop RT PCR (TaqMan MicroRNA Assays; Applied Biosystems) was used to quantitate microRNAs according to the earlier published conditions (Ichimi et al, 2009).
Stem-loop RT PCR (TaqMan MicroRNA Assays; P/N: PM10617 for miR-1, and PM10413 for miR-133a; Applied Biosystems) was used to quantitate miRNAs according to the earlier published conditions (Ichimi et al, 2009).
TNFα was quantitated by ELISA according to the manufacturer's protocol (BD Biosciences, San Diego, CA, USA).
Purified cRNA was quantitated, chemically fragmented according to Affymetrix protocol, and then hybridized overnight on Human Genome U95A Arrays (which contain probe sets for the detection of approximately 12,600 transcripts).
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