In conjunction with the ratio of septal curvature, the relationship between the left ventricle and the right ventricle can be quantitated on the basis of the ratio between the LV antero-posterior dimension and the septo-lateral dimension at end systole or end diastole.
Symptoms were observed at 1 week post-inoculation and lesions quantitated on inoculated leaves using an AlphaInnotech imaging system.
Each gene amplicon was purified with the MiniElute PCR Purification Kit (Qiagen) and quantitated on an agarose gel by using a DNA mass ladder (Promega).
T-bet, GATA3, RORC, IFNγ, IL-9, IL-10, IL-13, IL-17A, and IL-17F transcripts were quantitated on single CD4+ T cells.
DNA from peripheral blood mononuclear cells was isolated using the QIAamp DNA mini kit (Qiagen) and quantitated on the NanoDrop 1000 spectrophotometer (Thermo Fischer Scientific).
The biotin-labeled cRNA was purified using the GeneChip Sample Cleanup Module and quantitated on a spectrophotometer and analyzed on a 1% agarose TAE gel to check for a wide distribution of cRNA transcript sizes.
Protein levels in individual pancreata were then quantitated on a Molecular Imager FX densitometer (Bio-Rad) using Quantity One 4.6 software (Bio-Rad) and normalized to the quantity of β-actin obtained for each sample.
Survival in these strains, quantitated on the basis of DIC appearance rather than fluorescence, was only 3 % and 8, respectively, comparable to the survival of stationary phase wild type cells (Table 1).
After hybridization and washing of the Southern blot followed by autoradiography, the relative intensity of hybridization in the intact p53 allele (20 kb), the excised p53 allele (10 kb) and a p53 pseudogene (2 kb) was quantitated on a GE Molecular Dynamics phosphorimager.
