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Such a pattern has been demonstrated under ecological speciation in nature but is notoriously difficult to quantitate (for example [ 43- 46]).
Although methods for semi-quantification have been proposed [ 38, 39], HSQC-NMR inherentlytly difficult to quantitate for a polymer system for a variety of reasons, as outlined by Zhang et al [ 40].
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The appearance of proliferating colonies of cells was monitored and quantitated for up to 4 weeks.
The plasma samples of the patients (n = 53) were quantitated for the major thyroid hormones.
Further the cells were quantitated for expansion and apoptosis by various methods.
The kinetics and extent of uptake was quantitated for G-28 and U87 cell lines by flow cytometry (Fig. 1A).
Fluorescence intensity was determined using the integrated intensity function of MetaVue software; at least 150 cells were quantitated for each sample and each experiment was repeated 3 times.
Ten µl samples of each lysate were quantitated for total protein using the BioRad Protein Assay Dye Reagent (BioRad Laboratories, Hercules, CA).
The CD31-positive areas were quantitated for each random field as % of total stained area by pixel counting using the 'SimplePCI' imaging software (Hamamatsu Corporation, Sewickley, PA).
But also in the bacterial domain of life, the number of genome copies has only be quantitated for a limited number of species.
The percent of unbound, labeled sodB promoter was quantitated for each competition EMSA using densitometry as a means of comparing the relative affinity of each promoter fragment for Fur.
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