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The simulations prove invaluable in quantifying, using performance-based design concepts, benefits of proposed seismic retrofits schemes, prior to embarking on expensive procedures.
The principle for copy number quantifying using qPCR was described in previous study [ 77].
Gels were dried and incubated with phosphor-storage screens for scanning in Typhoon 9400 (Amersham Biosciences) and quantifying using ImageQuant software (Amersham Biosciences).
The products were detected in a 1% (v/v) agarose 1 × TAE gel and stained with ethidium bromide before quantifying using the Gene Snap (SynGene) program.
A standard curve was produced by cloning the near full-length 16S rRNA gene of Vibrio natriegens DSMZ 759 amplified using primers 27 F and 1492R [ 64] into the TOPO TA vector (Life Technologies, Carlsbad, California, USA), quantifying using fluorescent assay (Quant-IT, Life Technologies) and diluting to produce a standard curve from 10to1010 copies per μl.
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Data were quantified using MetaXpress software.
All images were quantified using ImageJ (NIH).
NRF2 images were quantified using ImageJ.
FRET measurements were quantified using ImageJ (NIH).
Diversification through time is often quantified using simple taxonomic methods.
siRNA concentration was quantified using QuantIT PicoGreen assay.
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