Based on this observation, we have developed a supercoiling-sensitive quantitative PCR assay (ss-qPCR) to quantify oxidative damage in the supercoiled DNA [ 34, 37].
ELISA for 8-OHdG, a recognized marker of oxidative DNA damage, was used to quantify oxidative DNA damage in Caco-2/15 cells.
It is also used to quantify oxidative DNA damage, alkali-labile sites, DNA DNA or DNA protein cross-links and abasic sites [56, 57, 58].
For each biopsy sample, mtDNA was quantified relative to nuclear DNA (mtDNA content) by qPCR, mtDNA deletions were investigated by long-template PCR followed by gel densitometry, and mtDNA oxidative damage was quantified using a qPCR-based assay.
Oxidative damage was determined by quantifying nitrotyrosine content.
We then quantified the carbonyl protein content, which is a marker for irreversible oxidative damage to proteins.
To assess oxidative damage by reactive oxygen species to islet proteins, the presence of protein carbonyl groups was quantified using the OxyBlotTM Protein Oxidation Detection Kit (Chemicon International, Temecula, CA, USA).
Oxidative damage biomarkers results.
Oxidative damage in the YSG Youngg Sedentary Group, YTG Young Trained Group, MSG Middle-Aged Group and MTG Middle-Aged Middle-AgedAged Trained Group).
However, ROS generation induced no oxidative damage.
For many reasons, the brain is highly vulnerable to oxidative damage.
