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SOD is one of several anti-oxidant enzymes that control concentrations of reactive oxygen species and catalyzes the dismutation of the superoxide radical (O2 −) (reviewed by [22]; (e) the immune system by quantifying expression of transferrin variant d (tfd) which encodes an iron transporter that can deprive harmful microorganisms of this essential nutrient [51].
As expected, mouse cell proportion in PDXs -evaluated by quantifying expression of the housekeeping gene TBP- correlated with all mouse endothelial markers and human VEGFA RNA levels.
Systematic studies of HERV expression patterns across tissues have been limited to quantifying expression of single elements or collective expression of entire HERV families [ 5, 7, 21- 23].
To determine the molecular basis of ovotestis and ovary development in these mutant lines we began by carefully quantifying expression of Sox9 during the sex-determining period of gonadogenesis, ∼11.25 12.5 dpc, using qRT-PCR.
We examined XCI in female lymphoblastoid cells by quantifying expression of XIST, the master gene for XCI (Brown et al., 1992), and other noncoding RNA genes that are involved in the regulation of XIST expression such as TSIX (Migeon et al., 2002), FTX (Chureau et al., 2002), and JPX (Chureau et al., 2002).
This practice not only discards potentially useful information, but also introduces an underestimation bias for quantifying expression of genes with highly redundant sequences (e.g., young duplicated genes).
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We validated our technique by quantifying expressions of these 5 genes starting from tissue samples of colorectal carcinoma and the surrounding normal mucosa of 33 different patients.
Real time RT-PCR was used to quantify expression of nine differentially expressed genes identified by microarray analysis.
Our objective was to identify signal transduction pathways expressed in a model of FAS and to quantify expression of c-fos, a gene in the stress signal pathway.
To confirm NRF2 was active in the nucleus, we quantified expression of two NRF2-regulated antioxidant response element (ARE) genes, NQO1 and HMOX1.
Furthermore, we quantified expression of the Soi-PBAN mRNA in the head, thorax and abdomen of the fire ant.
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