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This technique uses high-performance PCR to quantify up to 45 transcripts in small human DNA samples.
The new DNA quantification kits use multiplex TaqMan® assay-based fluorescent probe technology to simultaneously quantify up to three human genomic targets, allowing samples to be assessed for total human DNA, male contributor (i.e., Y-chromosome) DNA, as well as a determination of DNA degradation state.
As shown in [6], [7], state-of-the-art mass spectrometry can only quantify up to ∼5000 proteins and has a tendency to assay relatively highly expressed proteins.
Multiple cytokine and chemokine assays are multiplex bead-based assays able to simultaneously quantify up to 32 (or even more) targets in one sample.
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The related substances could be quantified up to the 0.1% (w/w) level.
Similar efforts to date resulted in assays quantifying up to five analogues in various combinations [15 18] but none included the primary forms of vitamin D, 3-epi-25OHD2 or 1α25(OH)2D2.
This study quantifies up to a 12.1% decrease in the burning rate constant of Kerosene droplets when 6.1 wt% nanoaluminum (nAl) particles are added (the maximum stable loading) with a hydrocarbon-based surfactant in a free-falling single droplet combustion experiment.
Urine output and electrolyte contents were then quantified up to 5 h and compared with those administered with furosemide 10 mg/kg (F10) and distilled water (CON).
A total of 19 variants induced aberrant splicing and we identified and quantified up to 12 different aberrant RNA isoforms from minigenes of exons 19-20 and 23-24.
This system is a rapid multiplexed assay platform that quantifies up to 100 distinct analytes simultaneously in a single sample in a 96-well plate format.
The nCounter® system is highly multiplexed, with the capacity of quantifying up to 800 targets simultaneously, and has been shown to be more sensitive than microarrays and similar in sensitivity to real-time PCR [ 19, 20].
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