Sentence examples for quantify the turnover of from inspiring English sources

Here flow cytometry and FACS were used to quantify the turnover of GFP‐LC3 (reflecting an autophagic flux) as a reliable and simple assay to measure autophagic activity in living mammalian cells.

Shvets et al. more recently pioneered the use of flow cytometry to quantify the turnover of GFP-LC3 as an assay to measure autophagic activity in living mammalian cells [ 7].

The 6 h incubation time used to quantify acute, follicular GC metabolism at the time of OR was based on the outcome of a series of 10 replicate, individual patient validation cultures and reflected the minimal time required to accurately quantify the turnover of all three metabolites by 1 × 10 viable GCs in vitro.

To quantify the turnover rates of MHET and BHET, reactions (100 µL) with 1 mM of the substrate in 40 mM NaPi pH 7.5 with 80 mM NaCl and 20% (v/v) DMSO were incubated at 30 °C before they were stopped and analyzed as described above.

To more accurately quantify the turnover rate of Bax, a classical 35S-methionine pulse chase method was also employed.

Here, we selected only individuals with a particularly good health status and used in vivo labeling with deuterated water (H2O) to quantify the turnover rates of naive, memory, and natural effector B cells, naive and memory CD4+ and CD8+ T cells, and γδ T cells in young and elderly healthy individuals.

DOI: http://dx.doi.org/10.7554/eLife.02555.018 To delineate the requirement of Smurfs for Shh-induced Ptch1 turnover, we took the advantage of the conditional Smurf1 −/− ;Smurf2 fl/fl MEFs, and quantified the turnover rate of exogenously expressed Ptch1-FLAG following cyclohexamide treatment without or with removal of the Smurf2 alleles following Ad-cre infection.

We further quantified the turnover rate of Ptch1 in Smonull cells, and found that Shh still promotes Ptch1 turnover there (Fig.8G, 8H).

We quantify the level of turnover of tRNA genes across the genus Drosophila and propose the existence of core and peripheral sets of tRNA genes.

Second, flux through the pathway is quantified using radioisotopes to trace the turnover of a single metabolite, typically the end product of the pathway.

We averaged the values of properties that quantify the relative variability of mRNA abundance [ 13], turnover of H3 histone promoter [ 15], sensitivity to regulators [ 14], expression noise [ 34], nucleosome measurability, and nucleosome SNR.