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These approaches include amplification of both the 16S rRNA gene and functional genes to identify specific microbes and qPCR to quantify target genes (Smith and Osborn2009).
The ability to accurately detect and quantify target DNA in mixed-species samples is crucial when target DNA may be overwhelmed by non-target DNA.
As a result, a great number of MBs are opened that is associated with a dramatically amplified fluorescent signal, enabling to quantify target DNA down to 100 fM.
To assess rcDNA degradation, we suggest two quantitative real-time PCR assays designed to separately quantify target sequences shorter and longer than the 200 bp risk limit, the relative abundance of both targets reflecting the extent of rcDNA fragmentation.
A number of acquisition constellations for airborne or spaceborne optical images involve small time-lags and produce near-simultaneous images, a type of data which has thus far been little exploited to detect or quantify target motion at the Earth's surface.
Data collection was carried out with the nCounter Digital Analyzer to count individual fluorescent barcodes and quantify target RNA molecules present in each sample.
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New strategy for designing primers based on partial 16S rRNA genes to quantify targeted uncultured bacteria was successfully developed.
The comparative CT method was used to quantify targets; CT values were normalized to β-actin levels in each tissue and expressed relative to the indicated target in the indicated tissues.
Changes in current voltage characteristics were used to identify and quantify targets that hybridize with specific complementary probes covalently functionalized on the membrane surface.
With the aid of UPLC-UV-MS, we were able to quantify targeted pigments and identify unexpected compounds in Dunaliella samples (see details in " Materials and methods" section).
Previously, we have shown that under steady-state conditions, an enzyme that is capable of acetylating two or more locations or residues on the same substrate will have an impact on the ability to obtain an accurate measurement of Km or kcat, but kcat/ Km (or kcat/ K1/2 nH ) is the correct value to quantify targeting of a given site.
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