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Custom visual basic programs integrated MS peaks and applied the 5PL fits to quantify standards and samples (Fig. 3d).
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The performance was measured against a quantified standard HBV DNA working reagent (NIBSC code 98/780) and the sensitivity compared with our conventional thermal-block PCR.
In the present study we evaluated the therapeutic potential of a ketogenic diet directly by quantifying standard measures of pain and inflammation in juvenile and adult rats.
Lipids, expressed as ratios to the relevant internal standard, were quantified using standard curves prepared with sphingomyelin, galactosylceramide, and sulfatide external standards (Avanti Polar Lipids).
HPLC chromatograph peaks were identified by comparing their retention times and absorption spectra to authentic standards, and quantified using standard curves corresponding to carotenoid compounds.
To quantify signals, standard compounds of the observed metabolites were prepared at 2, 5, and 10 mM.
t-PA, PAI-1 and fibrin fragment D-dimer antigen levels were quantified using standards commercial ELISA methods.
Compounds were identified and quantified using standards.
Samples were read at 405 nm on an ELISA plate reader and quantified using standards supplied by the manufacturer.
Because internal standards were not available, monoPAPs and diPAPs were quantified by standard addition.
RWE-specific IgE was quantified using standard sandwich ELISA technique and comparison with an IgE standard curve as described previously[41].
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CEO of Professional Science Editing for Scientists @ prosciediting.com