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We designed primers and a dual labelled fluorescent probe that can be used to quantify numbers of P. hermaphrodita and which is capable of distinguishing this species from the morphologically identical Phasmarhabditis neopapillosa.
A methylene blue assay [21], [22] was used to quantify numbers of adherent viable cells.
The annexin V/propidium iodide (BD Clontech) was used to quantify numbers of apoptotic cells as described previously [44].
To quantify numbers of cells with epithelial and mesenchymal phenotypes in cultured cells, we employed flow cytometry and started by monitoring cells for the epithelial marker EpCAM as well as monitoring for vimentin or CD44 marking cells with mesenchymal attributes.
While the NSA model does not allow us to accurately quantify numbers of stem cells (due solely to fact that we do not know how many stem cells we started with), it does allow a comparison between groups of populations of the stem cell expansion rate, which reflects the frequency of symmetric stem cell division.
The Optical Fractionator Probe was used to quantify numbers of NeuN-ir cells.
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Using MODIS IST we quantify number of melt days and areal extent of melt for each year of the study.
Two parameters were quantified: numbers of neovessels with blind ends and neovessel integration with neighboring neovessels as measured by counting closed vascular loops (polygons) [18].
Four laboratories provided protocols for quantifying numbers of interferon-γ spot forming cells in human peripheral blood mononuclear cells stimulated with Mycobacterium tuberculosis derived antigens.
In addition, as suggested by the reviewer, we quantified numbers of Cux1 positive cells in relation to total cell number in both Gsk3 mutants and controls.
Smoking was quantified (number of cigarettes and years smoked), and smoking status was classified into current or nonsmokers.
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