This paper reports results from an experimental pilot study performed to quantify the manual dexterity of older Mexican American adults.
The method was validated by measuring different algorithmic and physiologic indices and quantified with manual segmentation ground truths, provided by a cardiologist.
Foci were quantified by manual counting and the Cell Profiler Software (www.cellprofiler.org).org
The number of K5 and K8 positive cells were quantified by manual counting of at least 100 cells in three random fields per experimental sample.
For motility measurements, cells were imaged every 15 minutes at 10× magnification for >6 hours and quantified using manual tracking in ImageJ.
The stained invasive cells were photographed under an inverted light microscope (100× magnification) and quantified by manual counting in three randomly selected areas.
Total lesion area was quantified by manual tracing of entire intimal lesion in 10 equally spaced aortic root sections per mouse.
Interaction between the male and female mice was quantified by manual annotation of video recordings in which the behaviors were categorized as sniff body, approach, chase, walk away, mount and attempt mount, thrust and/or ejaculation, or non-interacting.
Analysis of the track of each cell in the field is quantified by manual rendering of the nuclear profile in all frames allowing localization of the coordinates of the nucleus centroid and calculation of path length, displacement from origin, and average speed (total path length/time).
Images were quantified by manual counting using NIS-Elements (Nikon) software and images processed using Adobe Photoshop.
