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These studies most often quantify frequencies of AA and TT steps rather than individual di, tri, tetra, and penta-A and T-containing nucleotide motifs [7], [16], [29], [30].
(L ) Classification system used to quantify frequencies of mutant phenotypes.
To quantify frequencies of cells expressing each shRNA, we isolated genomic DNA from the sorted populations and then PCR-amplified, purified and analyzed by deep-sequencing the shRNA-encoding cassettes.
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We used Illumina sequencing to quantify the frequencies of mutations before and after selection for viral growth.
This is consistent with the observed reduction in inflammatory cytokine production, although the authors did not quantify the frequencies of M1 and M2 macrophages.
DOI: http://dx.doi.org/10.7554/eLife.08474.004 We employed deep-sequencing to map and quantify the frequencies of independent insertion sites of the retroviral gene-trap in selected cells and compared this with the landscape of insertions in unselected control cells.
The Craig Hospital Eye Evaluation Rating Scale (CHEERS) was designed to systematically quantify frequency and severity of eye movement deficits in TBI.
As a control, we quantified the frequencies of BV6+ cells in the thymus of neonatal B6 mice.
Others have focused on quantifying the frequencies of subcomponents of isoforms, such as individual exons (Kakaradov et al., 2012; Katz et al., 2010).
DOI: http://dx.doi.org/10.7554/eLife.02669.005 To assess the persistence of NICs in vivo we quantified the frequencies of all SVZ cells, GEPCOTs, and neurospheres that were tdTomato+ at 2, 7, 28, or 60 days after tamoxifen treatment.
We determined whether similar mutational mechanisms act upon segmental duplications as in non-duplicated genomic regions by quantifying the frequencies of DNA oligomers at DIMs and high-quality SNPs.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com