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PDQuest 2D image software was used to quantify DEPs of each NFPA subtype relative to controls.
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One method of quantifying the effectiveness of the DEP trap is the use of light intensity measurements.
Nobody can quantify any of this stuff.
Laboratories quantify the threshold of trust.
* Total number of DEPs (number of upregulated DEPs/number of downregulated DEPs).
Furthermore, DEP spectra can offer information on the optimal frequencies for separating vancomycin treated cells from untreated cells of each C. difficile strain, thereby enabling a means for quantifying the efficacy of vancomycin treatment on each strain, especially within heterogeneous C. difficile samples.
After characterization of isolated mitochondria, mitochondrial differentially expressed proteins (DEPs) were quantified by two-dimensional difference in-gel electrophoresis (2D-DIGE), and identified by peptide mass fingerprint (PMF) and tandem mass spectrometry (MS/MS).
In fact, the limitations of this method coming from time consuming procedures, limited throughput and the complex physical phenomena of unknown cell properties (varying DEP forces as a cell moves make it difficult to quantify).
Analyzing the impact of loss of DEP-1 function, induction of collateralization was quantified after CCAO and hindlimb femoral artery ligation comparing wild-type and DEP-1−/− mice.
However, these particles did not necessarily reflect the effects of DEP representative of present diesel automobiles.
Kevin John Selwyn Knott, LVO, Dep Sec of the Duchy of Cornwall and Dep Treasurer to The Prince of Wales.
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