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The performance was measured against a quantified standard HBV DNA working reagent (NIBSC code 98/780) and the sensitivity compared with our conventional thermal-block PCR.
Ten-fold serial dilutions of the purified and quantified standard were included in the experiment to create the standard curve in order to know the number of initial DNA molecules in the samples.
Tenfold serial dilutions of a purified and quantified standard were included in the experiments to determine the standard curve in order to estimate the initial number of DNA molecules in each sample.
Although no quantified standard was available, relative estimation of syndecan-2 and syndecan-4 levels was possible in a small pilot material.
Each microtitre plate included a serial dilution of quantified standard DNA containing 10 fg to 10 ng.
LDA is simple to conduct, is independent of the kinetic and optical parameters upon which real-time qPCR is founded, does not require a quantified standard and is intrinsically self validating.
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In the present study we evaluated the therapeutic potential of a ketogenic diet directly by quantifying standard measures of pain and inflammation in juvenile and adult rats.
Custom visual basic programs integrated MS peaks and applied the 5PL fits to quantify standards and samples (Fig. 3d).
Lipids, expressed as ratios to the relevant internal standard, were quantified using standard curves prepared with sphingomyelin, galactosylceramide, and sulfatide external standards (Avanti Polar Lipids).
HPLC chromatograph peaks were identified by comparing their retention times and absorption spectra to authentic standards, and quantified using standard curves corresponding to carotenoid compounds.
RWE-specific IgE was quantified using standard sandwich ELISA technique and comparison with an IgE standard curve as described previously[41].
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