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VH was quantified relative to the internal standard PVH (ratio between peak areas).
Glycogen was extracted from G. sulphuraria cells as previously described (Martinez-Garcia et al. 2016) and was quantified relative to the dry biomass.
They were quantified relative to a mixture of internal standards.
The signal was quantified relative to the input material used in the first ChIP.
Samples were quantified relative to a 3D6 antibody standard of known concentration.
The immunoprecipitated material was quantified relative to a standard curve of genomic DNA.
mRNA gene-expression was quantified relative to either GAPDH or 18 s rRNA housekeeping genes.
The amount of immunoprecipitated DNA was quantified relative to the amount of the input DNA for each sample.
Phosphorylated p38/MAPK (pp38) levels were quantified relative to total p38/MAPK protein levels, with Scion software.
The intensity of the YM1 signal was quantified relative to that of β-actin using image J software.
In this analysis, each individual lipid in the PD samples was quantified relative to the levels detected in Con samples.
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